Role of denture-wearing on colonization and antifungal resistance of oral Candida albicans isolates in healthy people

The primary focus of the present study was to evaluate the occurrence of Candida albicans isolatesin the oral cavity and its probable correlation with dental implant applications and prosthesis. Wecollected oral swabs from patients who had attended private dentistry clinics, followed by stringentand controlled antifungal susceptibility testing and calculation of colony forming units (CFUs).Amphotericin B, Fluconazole and Itraconazole were three antifungals tested by CLSI M27-A2 brothmicrodilution protocol. The MIC ranges for three tested antifungals in Candida albicans isolateswere obtained as 0.0625-1, 0.125-16 and 0.0313-0.5 μg/ml, respectively. Additionally, in non-Candida albicans isolates, MIC ranges for three antifungals were achieved as 0.25-1, 0.125-16 and0.0313-0.125 μg/ml, respectively. MIC50 values of both tested azoles in the Candida albicansgroupwere higher than related values in the non-Candida albicans group. Moreover, CFU counts fordenture-acquired people were higher than for participants not wearing denture applications,indicating the proposal that the surface of dentures or any other synthetic implants in the oral cavitymay result in providing an appropriate environment for the colonization of yeasts.